Noncovalent and site-directed spin labeling of duplex RNA.
نویسندگان
چکیده
An isoindoline-nitroxide derivative of guanine (Ǵ, "G-spin") was shown to bind specifically and effectively to abasic sites in duplex RNAs. Distance measurements on a Ǵ-labeled duplex RNA with PELDOR (DEER) showed a strong orientation dependence. Thus, Ǵ is a readily synthesized, orientation-selective spin label for "mix and measure" PELDOR experiments.
منابع مشابه
Purine-Derived Nitroxides for Noncovalent Spin-Labeling of Abasic Sites in Duplex Nucleic Acids.
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عنوان ژورنال:
- Chemical communications
دوره 52 100 شماره
صفحات -
تاریخ انتشار 2016